DEF6 Antibody

Western Blot: DEF6 Antibody [NB300-837] - Analysis of DEF6 on Human Thymus lysate using NB300-837 at 1ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DEF6 Antibody Summary

• Description: Novus Biologicals Goat DEF6 Antibody (NB300-837) is a polyclonal antibody validated for use in WB and ELISA. Anti-DEF6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence ALRKELLKSIWYA-C corresponding to N-Terminus according to NP_071330.3.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DEF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human thymus lysates (calculated MW of 73.9 kDa band according to NP_071330.3).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DEF6 Antibody

• DEF-6
• differentially expressed in FDCP 6 homolog (mouse)
• differentially expressed in FDCP 6 homolog
• IBPdifferentially expressed in FDCP (mouse homolog) 6
• IRF4-binding protein
• SLAT
• SWAP70L
• SWAP-70-like adaptor protein of T cells

Background

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEF6 Antibody (NB300-837)(1)

Publication using NB300-837

• Tanaka Y, Bi K, Kitamura R et al. SWAP-70-like Adapter of T Cells, an Adapter Protein that Regulates Early TCR-Initiated Signaling in Th2 Lineage Cells. Immunity 2003-03-01 [PMID: 12648457]

Bioinformatics

• Gene Symbol: DEF6
• Entrez:
  • 50619(Human)
  • 23853(Mouse)
• Uniprot:
  • NP_071330.(Human)