DEF6 Antibody (1F2) - Azide and BSA Free

Immunohistochemistry-Paraffin: DEF6 Antibody (1F2) [H00050619-M01] - Analysis of monoclonal antibody to DEF6 on formalin-fixed paraffin-embedded human lymph node Antibody concentration 3 ug/ml.

Sandwich ELISA: DEF6 Antibody (1F2) [H00050619-M01] - Detection limit for recombinant GST tagged DEF6 is approximately 1ng/ml as a capture antibody.

Immunoprecipitation: DEF6 Antibody (1F2) [H00050619-M01] - Analysis of DEF6 transfected lysate using anti-DEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DEF6 monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 1F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DEF6 Antibody (1F2) - Azide and BSA Free Summary

• Immunogen: DEF6 (AAH17504, 1 a.a. ~ 336 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALRKELLKSIWYAFTALDVEKSGKVSKSQLKVLSHNLYTVLHIPHDPVALEEHFRDDDDGPVSSQGYMPYLNKYILDKVEEGAFVKEHFDELCWTLTAKKNYRADSNGNSMLSNQDAFRLWCLFNFLSEDKYPLIMVPDEVEYLLKKVLSSMSLEVSLGELEELLAQEAQVAQTTGGLSVWQFLELFNSGRCLRGVGRDTLSMAIHEVYQELIQDVLKQGYLWKRGHLRRNWAERWFQLQPSCLCYFGSEECKEKRGIIPLDAHCCVEVLPDRDGKRCMFCVKTATRTYEMSASDTRQRQEWTAAIQMAIRLQAEGKTSLHKDLKQKRREQREQR
• Specificity: DEF6 - differentially expressed in FDCP 6 homolog (mouse)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DEF6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DEF6 Antibody (1F2) - Azide and BSA Free

• DEF-6
• differentially expressed in FDCP 6 homolog (mouse)
• differentially expressed in FDCP 6 homolog
• IBPdifferentially expressed in FDCP (mouse homolog) 6
• IRF4-binding protein
• SLAT
• SWAP70L
• SWAP-70-like adaptor protein of T cells

Background

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEF6 Antibody (H00050619-M01)(2)

Publications using H00050619-M01

• Liew PL, Fang CY, Lee YC et al. DEF6 expression in ovarian carcinoma correlates with poor patient survival. Diagn Pathol 2016-08-03 [PMID: 27488395]
• Worthmann K, Leitges M, Teng B et al. Def-6, a Novel Regulator of Small GTPases in Podocytes, Acts Downstream of Atypical Protein Kinase C (aPKC) L/I. Am J Pathol. 2013-10-03 [PMID: 24096077]

Bioinformatics

• Gene Symbol: DEF6
• Entrez:
  • 50619(Human)