DEC1 Antibody (5B1) - Azide and BSA Free

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHB2 expression in NIH/3T3.

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - Western Blot analysis of BHLHB2 expression in Hela NE ( Cat # L013V3 ).

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1 Analysis of BHLHB2 expression in HepG2.

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHB2 expression in Raw 264.7.

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHE40 expression in K-562.

ELISA: DEC1 Antibody (5B1) [H00008553-M01] - Detection limit for recombinant GST tagged BHLHB2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 5B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DEC1 Antibody (5B1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: BHLHB2 (NP_003661, 130 a.a. ~ 229 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LSGRNVETGQEMFCSGFQTCAREVLQYLAKHENTRDLKSSQLVTHLHRVVSELLQGGTSRKPSDPAPKVMDFKEKPSSPAKGSEGPGKNCVPVIQRTFAH
• Specificity: BHLHB2 - basic helix-loop-helix domain containing, class B, 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BHLHE40
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DEC1 Antibody (5B1) - Azide and BSA Free

• basic helix-loop-helix domain containing, class B, 2
• basic helix-loop-helix family, member e40
• bHLHb2
• bHLHe40
• Class B basic helix-loop-helix protein 2
• class E basic helix-loop-helix protein 40
• DEC1HLHB2
• differentially expressed in chondrocytes 1
• Differentially expressed in chondrocytes protein 1
• differentiated embryo chondrocyte expressed gene 1
• Enhancer-of-split and hairy-related protein 2
• SHARP2
• SHARP-2
• Stimulated by retinoic acid gene 13 protein
• STRA13FLJ99214
• Stra14

Background

DEC1 encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. Differentiated embryo chondrocyte expressed gene 1 is believed to be involved in the control of cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEC1 Antibody (H00008553-M01)(2)

Publications using H00008553-M01

• Krutilina R, Sun W, Sethuraman A et al. MicroRNA-18a inhibits hypoxia-inducible factor 1-alpha activity and lung metastasis in basal breast cancers. Breast Cancer Res. 2014-07-28 [PMID: 25069832]
• Lecomte V, Meugnier E, Euthine V et al. A new role for sterol regulatory element binding protein 1 transcription factors in the regulation of muscle mass and muscle cell differentiation. Mol Cell Biol 30(5):1182-98. 2010-03-01 [PMID: 20028734]

Bioinformatics

• Gene Symbol: BHLHE40
• Entrez:
  • 8553(Human)
• OMIM:
  • 604256(Human)
• Uniprot:
  • NP_003661(Human)