DDX47 Antibody

Western Blot:[NBP3-30439]-Detection of human DDX47 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-DDX47 antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 3 µg/mg lysate. DDX47 was also immunoprecipitated by rabbit anti-DDX47 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes and 10 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DDX47 Antibody Summary

• Description: Novus Biologicals Rabbit DDX47 Antibody (NBP3-30439) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: between 1 and 50
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX47
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-buffered Saline 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for DDX47 Antibody

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
• DEAD box polypeptide 47
• DEAD box protein 47
• DKFZp564O176
• E4-DBP
• E4-DEAD box protein
• EC 3.6.1
• EC 3.6.4.13
• FLJ30012
• HQ0256
• MSTP162
• probable ATP-dependent RNA helicase DDX47
• RRP3

Background

DDX47 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX47