DDX47 Antibody

Western Blot: DDX47 Antibody [NBP2-16139] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP2-16139] - Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using antibody at 1:250 dilution.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DDX47 Antibody Summary

• Description: Novus Biologicals Rabbit DDX47 Antibody (NBP2-16139) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DDX47. The exact sequence is proprietary.
• Localization: Nucleus; nucleolus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX47
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DDX47 Antibody

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
• DEAD box polypeptide 47
• DEAD box protein 47
• DKFZp564O176
• E4-DBP
• E4-DEAD box protein
• EC 3.6.1
• EC 3.6.4.13
• FLJ30012
• HQ0256
• MSTP162
• probable ATP-dependent RNA helicase DDX47
• RRP3

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DDX47
• Uniprot:
  • Q9H0S4(Human)