DDX3 Antibody

Western Blot: DDX3 Antibody [NBP1-85290] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DDX3X antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP1-85290] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Western Blot: DDX3 Antibody [NBP1-85290] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in germinal center cells.

Product Discontinued

DDX3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX3X
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DDX3 Antibody

• DBXCAP-Rf
• DDX14
• DDX3DEAD/H box-3
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• DEAD box protein 3, X-chromosomal
• DEAD box, X isoform
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3
• EC 3.6.1
• EC 3.6.4.13
• helicase like protein 2
• Helicase-like protein 2
• HLP2ATP-dependent RNA helicase DDX3X

Background

DDX3X, also known as ATP-dependent RNA helicase DDX3X, consists of a 73.2 kDa and a 71.4 kDa isoform which is involved in multiple gene expression steps, including transcription and translation. Current research is linking the protein to diseases and disorders such as hypoxia, hepatitis, malaria, infertility, carcinoma, breast cancer, and prostate cancer. The protein interacts in translational control, cell cycle checkpoint control, and receptor signaling pathways with other proteins such as ACTB, TBK1, NFKB2, NXF1, and EIF3B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX3X