DDX3 Antibody

Product Discontinued

DDX3 Antibody Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DDX3. The immunogen is located within amino acids 110 - 160 of DDX3.
• Specificity: DDX3 antibody will detect both DDX3 and DBY.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX3X
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 - 1 mg/ml. DDX3 antibody will detect both DDX3 and DBY.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX3 Antibody

• DBXCAP-Rf
• DDX14
• DDX3DEAD/H box-3
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• DEAD box protein 3, X-chromosomal
• DEAD box, X isoform
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3
• EC 3.6.1
• EC 3.6.4.13
• helicase like protein 2
• Helicase-like protein 2
• HLP2ATP-dependent RNA helicase DDX3X

Background

DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX3X
• Uniprot:
  • AAC34298(Human)