DcTRAILR2/TNFRSF22 Antibody (263310) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB
• Clone: 263310
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

DcTRAILR2/TNFRSF22 Antibody (263310) [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse DcTRAIL R2/TNFRSF22
  Ala39-Arg175
  Accession # Q9ER62
• Specificity: Detects mouse DcTRAIL R2/TNFRSF22 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB, rmBAFF R, rmCD27, rmCD30, rmCD40, rmDcTRAIL R1, rmDR3, rmEDAR, rmFas, rmGITR, rmHVEM
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DcTRAILR2/TNFRSF22 Antibody (263310) [Alexa Fluor® 532]

• APO-3;DDR3;DR3;GEF720;LARD;PLEKHG5;TNFRSF12;TNFRSF25;TR3;TRAMP;Tumor necrosis factor receptor superfamily member 25;WSL-1;WSL-LR
• DcTRAIL R2
• DcTRAILR2
• SOBa
• TNFRH2
• TNFRSF22

Background

TRAIL interacts via a complex receptor system to preferentially induce apoptosis of tumor cells in vivo and in vitro (1). In human, two signaling receptors (TRAIL R1 and TRAIL R2) and three decoy receptors (TRAIL R3, TRAIL R4, and OPG) for TRAIL exist. Mouse homologues of human TRAIL R1, -3, and -4 have not been found. Three novel mouse genes (TNF RH1, -2, and -3) that encode proteins with the classic TNF receptor cysteine-rich domains (CRDs) have been identified. These mouse genes are closely clustered on distal mouse chromosome 7 and do not have orthologues in the corresponding human chromosomal region (2). TNF RH1 and -2 have been implicated as decoy receptors for mouse TRAIL and named mDcTRAIL R1/TNFRSF23 and mDcTRAIL R2/TNFRSF22, respectively. The ligand for TNF RH3 is not known (1). The mouse decoy TRAIL R2 (mDcTRAIL R2) gene encodes a 198 amino acid (aa) type I transmembrane protein with a putative 39 aa signal peptide, a 136 aa extracellular domain, a 18 aa transmembrane region, and a 5 aa cytoplasmic tail (1, 2). An alternatively spliced variant encoding a secreted soluble receptor of 180 aa has also been described. The transmembrane and soluble receptors are known as mDcTRAIL R2_L and mDcTRAIL R2_S, respectively. Both mDcTRAIL R2 isoforms and the GPI-linked mDcTRAIL R1 bind TRAIL specifically but are incapable of triggering signal transduction. Mouse DcTRAIL R1 and mDcTRAIL R2 share 71% sequence identity (3). The two receptors have similar structural modules in their CRDs, which differ from those found in the CRDs of mTNF RH3 (2, 3). Mouse DcTRAIL R1 preferentially binds mTRAIL while mDcTRAIL R2 binds both human and mTRAIL. mRNA of mouse DcTRAIL R1 and -2 are expressed at low levels in many mouse tissues and at higher levels in several mouse cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9ER62()