DCP2 Antibody - BSA Free

Western Blot: DCP2 Antibody [NBP1-41070] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Affinity purified rabbit anti-Dcp2 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Dcp2 was also immunoprecipitated by another rabbit anti-Dcp2 antibody, which recognizes an upstream epitope.

• Reactivity: Hu, Pm-Or
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DCP2 Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 370 and 420 of human mRNA-decapping enzyme 2 using the numbering given in entry NP_689837.2 (GeneID 167227).
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 27918561)
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:1000 - 1:5000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DCP2 Antibody - BSA Free

• DCP2 decapping enzyme homolog (S. cerevisiae)
• EC 3.-
• FLJ33245
• hDpc
• mRNA-decapping enzyme 2
• nudix (nucleoside diphosphate linked moiety X)-type motif 20
• Nudix motif 20
• NUDT20Nucleoside diphosphate-linked moiety X motif 20

Background

DCP2 is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DCP2 Antibody (NBP1-41070)(8)

Publications using NBP1-41070

• Na Z, Luo Y, Cui DS et al. Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates Journal of the American Chemical Society 2021-08-18 [PMID: 34346674] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• Na Z, Luo Y, Schofield JA et al. The NBDY Microprotein Regulates Cellular RNA Decapping Biochemistry 2020-10-27 [PMID: 33059440] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• D'Lima NG, Ma J, Winkler L et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol 2017-02-01 [PMID: 27918561] (ICC/IF, WB, Human)
• Luo Y, Na Z, Slavoff SA Phage-display selection identifies a cell-permeable bicyclic peptide as a modulator of the human RNA decapping enzyme Dcp2 chemrxiv.org Jan 1 2020 (WB, Human)
• Luo Y, Na Z, Slavoff S Phage-Display Selection of a Cell-Permeable Bicyclic Peptide as a Selective Ligand of the Human RNA Decapping Enzyme Dcp2 ChemRxiv 2020-05-12 (WB, Human)
• Luo Y, Schofield JA, Simon MD, Slavoff SA Global Profiling of Cellular Substrates of Human Dcp2 Biochemistry 2020-05-14 [PMID: 32365300] (WB, Human)
• Luo Y, Na Z, Slavoff S Discovery of cellular substrates of human RNA decapping enzyme Dcp2 using a stapled bicyclic peptide inhibitor ChemRxiv Jul 20 2020 (WB, Human)
• Luo Y, Schofield JA, Na Z et al. Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor Cell chemical biology 2020-12-18 [PMID: 33357462]

Bioinformatics

• Gene Symbol: DCP2
• Entrez:
  • 167227(Human)