DC8 Antibody

Images

 
Western Blot: DC8 Antibody [NBP1-32281] - A. 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE DC8 antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: DC8 Antibody [NBP1-32281] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: DC8 Antibody [NBP1-32281] - Sample(30 ug of whole cell lysate)A:Hep G2 12% SDS PAGE, antibody diluted at 1:1000.
Western Blot: DC8 Antibody [NBP1-32281] - A: Hep G2 12% SDS PAGE diluted at 1:1000

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DC8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DC8. The exact sequence is proprietary.
Localization
Nucleus, Kinetochore
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NSL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DC8 Antibody

  • C1orf48
  • chromosome 1 open reading frame 48
  • DC8
  • DKFZp566O1646
  • kinetochore-associated protein NSL1 homolog
  • MIS14
  • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae)

Background

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DC8 Antibody (NBP1-32281) (0)

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol NSL1