DC8 Antibody

Western Blot: DC8 Antibody [NBP1-32281] - A. 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE DC8 antibody dilution: 1:1000

Immunocytochemistry/Immunofluorescence: DC8 Antibody [NBP1-32281] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Western Blot: DC8 Antibody [NBP1-32281] - Sample(30 ug of whole cell lysate)A:Hep G2 12% SDS PAGE, antibody diluted at 1:1000.

Western Blot: DC8 Antibody [NBP1-32281] - A: Hep G2 12% SDS PAGE diluted at 1:1000

Product Discontinued

DC8 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human DC8. The exact sequence is proprietary.
• Localization: Nucleus, Kinetochore
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for DC8 Antibody

• C1orf48
• chromosome 1 open reading frame 48
• DC8
• DKFZp566O1646
• kinetochore-associated protein NSL1 homolog
• MIS14
• NSL1, MIND kinetochore complex component, homolog (S. cerevisiae)

Background

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NSL1