DC-STAMP/TM7SF4 Antibody (788524) [Allophycocyanin]

Mature human monocyte-derived dendritic cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mLRecombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with 20 ng/mL LPS, 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA), and 20 ng/ml Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hourswere stained with Mouse Anti-Human CD83 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1774F) and either (A) Mouse Anti-Human DC-STAMP APC-conjugated Monoclonal Antibody (Catalog # FAB7824A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 788524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

DC-STAMP/TM7SF4 Antibody (788524) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human DC-STAMP
  Asp314-Thr376
  Accession # Q9H295
• Specificity: Detects human DC-STAMP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DC-STAMP is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCSTAMP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-STAMP/TM7SF4 Antibody (788524) [Allophycocyanin]

• DCSTAMP
• DC-STAMP
• dendritic cell-specific transmembrane protein
• FIND
• IL-4-induced Protein
• TM7SF4
• transmembrane 7 superfamily member 4

Background

DC-STAMP, also known as TM7SF4, is an approximately 50 kDa glycoprotein with seven transmembrane segments. It is expressed on the surface of dendritic cells and monocytes as well as on osteoclasts and their progenitors. DC-STAMP binds CTGF/CCN2 and cooperates with TRANCE/RANK L for inducing osteoclast differentiation and fusion into multinucleated cells. It contains a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and associates with FC gamma RIII/CD16. Within aa 314-376, human DC-STAMP shares approximately 75% aa sequence identity with mouse and rat DC-STAMP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DC-STAMP/TM7SF4 Antibody (FAB7824A)(1)

Publication using FAB7824A

• de la Rica L, Garcia-Gomez A, Comet N, Rodriguez-Ubreva J, Ciudad L, Vento-Tormo R, Company C, Alvarez-Errico D, Garcia M, Gomez-Vaquero C, Ballestar E NF-kappaB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation. Genome Biol, 2015-01-05;16(0):2. 2015-01-05 [PMID: 25601191] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: DCSTAMP
• Uniprot:
  • Q9H295(Human)