DAP5 Antibody (39C534.1)

Images

 
Western Blot: DAP5 Antibody (39C534.1) [NB120-11929] - Analysis of NB120-11929 (1ug) on 15 ug of total cell lysate from Jurkat cells.
Flow Cytometry: DAP5 Antibody (39C534.1) [NB120-11929] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using antibody (dark blue). Isotype control shown in orange. An antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow
Clone
39C534.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

DAP5 Antibody (39C534.1) Summary

Immunogen
The antibody was raised against two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein.
Specificity
In Jurkat, a 97 kDa band is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF4G2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1 ug per million cells
Positive Control
Jurkat Lysate (NB800-PC2)
DAP5 Lysate (NBL1-10210)
Publications
Read Publication using NB120-11929.

Reactivity Notes

Cross-reacts with Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for DAP5 Antibody (39C534.1)

  • aging-associated protein 1
  • DAP-5
  • DAP5AAG1
  • Death-associated protein 5
  • eIF4G 2
  • eIF-4G 2
  • eIF-4-gamma 2
  • eukaryotic translation initiation factor 4 gamma 2
  • eukaryotic translation initiation factor 4 gamma, 2
  • eukaryotic translation initiation factor 4G-like 1
  • FLJ41344
  • NAT1
  • p97

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini-protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAP5 Antibody (NB120-11929)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DAP5 Antibody (NB120-11929) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-11929AF405
Alexa Fluor 488 Labeled NB120-11929AF488
Alexa Fluor 647 Labeled NB120-11929AF647
Alexa Fluor 700 Labeled NB120-11929AF700
Allophycocyanin Labeled NB120-11929APC
Biotin Labeled NB120-11929B
DyLight 350 Labeled NB120-11929UV
DyLight 405 Labeled NB120-11929V
DyLight 488 Labeled NB120-11929G
DyLight 550 Labeled NB120-11929R
DyLight 650 Labeled NB120-11929C
DyLight 680 Labeled NB120-11929FR
DyLight 755 Labeled NB120-11929IR
HRP Labeled NB120-11929H
PE Labeled NB120-11929PE

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Bioinformatics

Gene Symbol EIF4G2
Entrez
Uniprot