DAP5 Antibody (39C534.1)

Western Blot: DAP5 Antibody (39C534.1) [NB120-11929] - Analysis of NB120-11929 (1ug) on 15 ug of total cell lysate from Jurkat cells.

Flow Cytometry: DAP5 Antibody (39C534.1) [NB120-11929] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Product Discontinued

DAP5 Antibody (39C534.1) Summary

• Immunogen: The antibody was raised against two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein.
• Specificity: In Jurkat, a 97 kDa band is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF4G2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug per million cells
  • Western Blot 1-2 ug/ml
• Control:

  Jurkat Whole Cell Lysate
  DAP5 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human and Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for DAP5 Antibody (39C534.1)

• aging-associated protein 1
• DAP-5
• DAP5AAG1
• Death-associated protein 5
• eIF4G 2
• eIF-4G 2
• eIF-4-gamma 2
• eukaryotic translation initiation factor 4 gamma 2
• eukaryotic translation initiation factor 4 gamma, 2
• eukaryotic translation initiation factor 4G-like 1
• FLJ41344
• NAT1
• p97

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini-protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAP5 Antibody (NB120-11929)(2)

Publications using NB120-11929

• Dave, P;George, B;Raheja, H;Rani, P;Behera, P;Das, S; The mammalian host protein DAP5 facilitates the initial round of translation of Coxsackievirus B3 RNA J. Biol. Chem. 2019-08-27 [PMID: 31455634] (WB, Human)
• Escobar, J, J W Frank et al. Amino acid Availability Age Affect the Leucine Stimulation of Protein Synthesis eIF4F Formation in Muscle: 00302. 2007-01-01 [PMID: 17878223]

Bioinformatics

• Gene Symbol: EIF4G2
• Entrez:
  • 1982(Human)
  • 13690(Mouse)
• Uniprot:
  • P78344(Human)