DAP12 Overexpression Lysate

Images

 
Western Blot: DAP12 Overexpression Lysate (Adult Normal) [NBL1-17484] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DAP12.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DAP12 Overexpression Lysate Summary

Description

DAP12 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC222502

Accession#: NM_003332

Protein Tag: C-MYC/DDK
Gene
TYROBP

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
9.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DAP12 Overexpression Lysate

  • DAP12
  • DAP12PLOSL
  • DNAX-activation protein 12
  • KARAP
  • KARAPPLO-SL
  • KAR-associated protein
  • killer activating receptor associated protein
  • Killer-activating receptor-associated protein
  • TYRO protein tyrosine kinase binding protein
  • TYRO protein tyrosine kinase-binding protein
  • TYROBP

Background

This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Two alternative transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described, but their full-length nature has not been deterimined. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB97861
Species: Hu
Applications: Flow, IHC, IHC-P
AF1729
Species: Mu
Applications: ELISA, ICC, KO, WB
DTRM10C
Species: Hu
Applications: ELISA
MAB139
Species: Hu
Applications: CostimT, CyTOF-ready, Flow, Neut, WB
H00010559-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-85959
Species: Hu
Applications: IHC, IHC-P
NBP1-32945
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
2249-NK
Species: Hu
Applications: BA
MAB2384
Species: Hu
Applications: CyTOF-ready, Flow, WB
7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
MAB1058
Species: Hu
Applications: Block, CyTOF-ready, Flow
462-TR/CF
Species: Mu
Applications: BA
NB100-56508
Species: Av, Hu, Mu, Rt
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P, WB
MAB1059
Species: Hu
Applications: CyTOF-ready, Flow
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NBL1-17484
Species: Hu
Applications: WB

Publications for DAP12 Lysate (NBL1-17484) (0)

There are no publications for DAP12 Lysate (NBL1-17484).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DAP12 Lysate (NBL1-17484) (0)

There are no reviews for DAP12 Lysate (NBL1-17484). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DAP12 Lysate (NBL1-17484) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DAP12 Products

Bioinformatics Tool for DAP12 Lysate (NBL1-17484)

Discover related pathways, diseases and genes to DAP12 Lysate (NBL1-17484). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DAP12 Lysate (NBL1-17484)

Discover more about diseases related to DAP12 Lysate (NBL1-17484).
 

Pathways for DAP12 Lysate (NBL1-17484)

View related products by pathway.

PTMs for DAP12 Lysate (NBL1-17484)

Learn more about PTMs related to DAP12 Lysate (NBL1-17484).
 

Research Areas for DAP12 Lysate (NBL1-17484)

Find related products by research area.

Blogs on DAP12.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DAP12 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol TYROBP