DAP12 Antibody (JG38-70)

Western Blot: DAP12 Antibody (JG38-70) [NBP2-76828] - Analysis of K562 cells with DAP12 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: DAP12 Antibody (JG38-70) [NBP2-76828] - Human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.

Western Blot: DAP12 Antibody (JG38-70) [NBP2-76828] - Analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.

Product Discontinued

DAP12 Antibody (JG38-70) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human DAP12 aa 1-113 / 113. (SwissProt: O43914 Human)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TYROBP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DAP12 Antibody (JG38-70)

• DAP12
• DAP12PLOSL
• DNAX-activation protein 12
• KARAP
• KARAPPLO-SL
• KAR-associated protein
• killer activating receptor associated protein
• Killer-activating receptor-associated protein
• TYRO protein tyrosine kinase binding protein
• TYRO protein tyrosine kinase-binding protein
• TYROBP

Background

DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells. DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction. Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice, as well as presenile dementia and bone cysts in humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TYROBP