DAP12 Antibody (406288) [Unconjugated]

Images

 
Western blot shows lysates of NS0 mouse myeloma cell line, mock transfected, and primary human CD56+ NK cells. PVDF membrane was probed with 5 µg/mL of Human DAP12 Monoclonal Antibody (Catalog # MAB5240) followed by ...read more
Human peripheral blood CD56+ natural killer cells were stained with Human DAP12 Monoclonal Antibody (Catalog # MAB5240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
406288
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

DAP12 Antibody (406288) [Unconjugated] Summary

Immunogen
Human Dap12 synthetic peptide
QGQRSDVYSDLNTQRPYYK
Accession # O43914
Specificity
Detects human DAP12 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TYROBP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB5240 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DAP12 Antibody (406288) [Unconjugated]

  • DAP12
  • DAP12PLOSL
  • DNAX-activation protein 12
  • KARAP
  • KARAPPLO-SL
  • KAR-associated protein
  • killer activating receptor associated protein
  • Killer-activating receptor-associated protein
  • TYRO protein tyrosine kinase binding protein
  • TYRO protein tyrosine kinase-binding protein
  • TYROBP

Background

DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB97861
Species: Hu
Applications: Flow, IHC, IHC-P
AF1729
Species: Mu
Applications: WB, ELISA, ICC, KO
MAB1278
Species: Hu
Applications: Flow, AgAct, CyTOF-ready
MAB139
Species: Hu
Applications: WB, Flow, CostimT, CyTOF-ready, Neut
NBP1-85959
Species: Hu
Applications: IHC, IHC-P
NBP1-32945
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
2249-NK
Species: Hu
MAB2384
Species: Hu
Applications: WB, Flow, CyTOF-ready
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
MAB1058
Species: Hu
Applications: Flow, Block, CyTOF-ready
NB100-56508
Species: Hu, Mu, Rt, Av
Applications: WB, Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
MAB1059
Species: Hu
Applications: Flow, CyTOF-ready
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
MAB5240
Species: Hu
Applications: WB, CyTOF-ready, Flow

Publications for DAP12 Antibody (MAB5240)(2)

Reviews for DAP12 Antibody (MAB5240) (0)

There are no reviews for DAP12 Antibody (MAB5240). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DAP12 Antibody (MAB5240) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional DAP12 Products

Bioinformatics Tool for DAP12 Antibody (MAB5240)

Discover related pathways, diseases and genes to DAP12 Antibody (MAB5240). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DAP12 Antibody (MAB5240)

Discover more about diseases related to DAP12 Antibody (MAB5240).
 

Pathways for DAP12 Antibody (MAB5240)

View related products by pathway.

PTMs for DAP12 Antibody (MAB5240)

Learn more about PTMs related to DAP12 Antibody (MAB5240).

Blogs on DAP12

There are no specific blogs for DAP12, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DAP12 Antibody (406288) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol TYROBP
Uniprot