DAP12 Antibody (406288) [Unconjugated]

Images

 
Western blot shows lysates of NS0 mouse myeloma cell line, mock transfected, and primary human CD56+ NK cells. PVDF membrane was probed with 5 µg/mL of Human DAP12 Monoclonal Antibody (Catalog # MAB5240) followed by ...read more
Human peripheral blood CD56+ natural killer cells were stained with Human DAP12 Monoclonal Antibody (Catalog # MAB5240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
406288
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

DAP12 Antibody (406288) [Unconjugated] Summary

Immunogen
Human Dap12 synthetic peptide
QGQRSDVYSDLNTQRPYYK
Accession # O43914
Specificity
Detects human DAP12 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TYROBP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 5 ug/mL
Publications
Read Publications using
MAB5240 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DAP12 Antibody (406288) [Unconjugated]

  • DAP12
  • DAP12PLOSL
  • DNAX-activation protein 12
  • KARAP
  • KARAPPLO-SL
  • KAR-associated protein
  • killer activating receptor associated protein
  • Killer-activating receptor-associated protein
  • TYRO protein tyrosine kinase binding protein
  • TYRO protein tyrosine kinase-binding protein
  • TYROBP

Background

DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAP12 Antibody (MAB5240)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DAP12 Antibody (MAB5240) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC5240U
Alexa Fluor 405 IC5240V
Alexa Fluor 488 IC5240G
Alexa Fluor 532 FAB5240X
Alexa Fluor 594 IC5240T
Alexa Fluor 647 IC5240R
Alexa Fluor 700 IC5240N
Alexa Fluor 750 IC5240S
Allophycocyanin FAB5240A
Biotin FAB5240B
DyLight 350 FAB5240D
DyLight 405 FAB5240E
DyLight 488 FAB5240K
DyLight 550 FAB5240L
DyLight 594 FAB5240M
DyLight 650 FAB5240W
DyLight 680 FAB5240Y
DyLight 755 FAB5240Z
FITC FAB5240F
HRP FAB5240H
Janelia Fluor 549 FAB5240I
Janelia Fluor 646 FAB5240J
PerCP FAB5240C
Phycoerythrin IC5240P

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Bioinformatics Tool for DAP12 Antibody (MAB5240)

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Blogs on DAP12.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

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Bioinformatics

Gene Symbol TYROBP
Uniprot