DAP12 Antibody (406209) [Unconjugated]

Human PBMCs were stained with (A) Human DAP12 Monoclonal Antibody (Catalog # MAB52401) or (B) isotype control antibody (MAB003), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B) and Rabbit anti-human CD56 PE-conjugated Monoclonal Antibody (FAB24086P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (1X) (FC005).

• Reactivity: Hu
• Applications: Flow
• Clone: 406209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DAP12 Antibody (406209) [Unconjugated] Summary

• Immunogen: Human Dap12 synthetic peptide
  Accession # O43914
• Specificity: Detects human DAP12 in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DAP12 Antibody (406209) [Unconjugated]

• DAP12
• DAP12PLOSL
• DNAX-activation protein 12
• KARAP
• KARAPPLO-SL
• KAR-associated protein
• killer activating receptor associated protein
• Killer-activating receptor-associated protein
• TYRO protein tyrosine kinase binding protein
• TYRO protein tyrosine kinase-binding protein
• TYROBP

Background

DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O43914(Human)