DAP Kinase 2 Antibody

Product Discontinued

DAP Kinase 2 Antibody Summary

• Immunogen: Peptide with sequence C-KALHPRRRSSTS corresponding to C-Terminus according to NP_055141.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DAPK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 40-45 kDa band bands observed in mouse brain extracts. IHC-P: Human breast shows staining of the luminar side of the cell membranes of epithelial cells in the ducts.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAP Kinase 2 Antibody

• DAP Kinase 2
• DAPK2
• DAP-kinase-related protein 1 beta isoform
• DAP-kinase-related protein 1
• death-associated protein kinase 2
• DRP1
• DRP-1
• DRP-1MGC119312
• EC 2.7.11
• EC 2.7.11.1

Background

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAP Kinase 2 Antibody (NB100-863)(2)

Publications using NB100-863

• Geering B, Stoeckle C, Rozman S et al. DAPK2 positively regulates motility of neutrophils and eosinophils in response to intermediary chemoattractants. J. Leukoc. Biol. 2014-02-01 [PMID: 24163421] (WB, Human)
  
  Details:
  Primary human leukocytes (Figs 1B-C), Recombinant DAPk2 (Fig 6A). Note: The specificity of antibody was validated with recombinant DAPk2 by WB (Fig 6A).
• Kawai T, Nomura F, Hoshino K et al. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999-06-10 [PMID: 10376525]

Bioinformatics

• Gene Symbol: DAPK2
• Entrez:
  • 23604(Human)
  • 13143(Mouse)
• Uniprot:
  • NP_055141.(Human)