DAN/NBL1 Antibody (123202)

Images

 
There are currently no images for DAN/NBL1 Antibody (MAB7551).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clone
123202
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

DAN/NBL1 Antibody (123202) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DAN
Ala17-Asp178
Accession # Q61477.2
Specificity
Detects mouse DAN in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) DAN, recombinant chicken Caronte, rhCerberus, or recombinant mouse Gremlin is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
NBL1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DAN/NBL1 Antibody (123202)

  • DAN domain family member 1
  • DAN
  • DAND1
  • MGC8972
  • NBL1
  • neuroblastoma suppressor of tumorigenicity 1
  • neuroblastoma, suppression of tumorigenicity 1
  • Protein N03
  • suppression of tumorigenicity 1
  • Zinc finger protein DAN

Background

DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in src-transformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP-2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA. It is likely the various DAN family members and other TGF-beta BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGF-beta superfamily ligands.

  1. Massage, J. and Y-G. Chen (2000) Genes & Development 14:627.
  2. Perch, J.J.H. et al. (1999) Develop. Biol. 209:98.
  3. Hsu, D.R. et al. (1998) Mol. Cell. 1:673.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1107
Species: Mu
Applications: IHC, WB
H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
AF2009
Species: Hu
Applications: ICC, IHC
NB200-109
Species: Hu, Mu
Applications: ChIP, CyTOF-ready, Flow, GS, ICC/IF, IP, WB
NBP2-67605
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-88220
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-98675
Species: Hu
Applications: IHC, IHC-P, IP, WB
NB100-1598
Species: Hu, Mu
Applications: Flow, ICC/IF, WB
NBP1-47974
Species: Ca, Hu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB600-534
Species: Hu, Mu, Po, V-Vi
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA, WB
NBP1-87581
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-15895
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-59787
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, IP, KD, PLA, WB
NB100-56598
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, KD, WB
NBP1-48002
Species: Ca, Hu, Pm, Pm
Applications: ICC/IF, IHC, IHC-P, IP, WB
5190-GR
Species: Hu
Applications: BA
AF2069
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), WB
AF1056
Species: Rt
Applications: IHC, WB

Publications for DAN/NBL1 Antibody (MAB7551) (0)

There are no publications for DAN/NBL1 Antibody (MAB7551).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DAN/NBL1 Antibody (MAB7551) (0)

There are no reviews for DAN/NBL1 Antibody (MAB7551). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DAN/NBL1 Antibody (MAB7551) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional DAN/NBL1 Products

Bioinformatics Tool for DAN/NBL1 Antibody (MAB7551)

Discover related pathways, diseases and genes to DAN/NBL1 Antibody (MAB7551). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DAN/NBL1 Antibody (MAB7551)

Discover more about diseases related to DAN/NBL1 Antibody (MAB7551).
 

Pathways for DAN/NBL1 Antibody (MAB7551)

View related products by pathway.

PTMs for DAN/NBL1 Antibody (MAB7551)

Learn more about PTMs related to DAN/NBL1 Antibody (MAB7551).

Blogs on DAN/NBL1

There are no specific blogs for DAN/NBL1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DAN/NBL1 Antibody (123202) and receive a gift card or discount.

Bioinformatics

Gene Symbol NBL1
Uniprot