DAGLA Antibody

Images

 
Western Blot: DAGLA Antibody [NBP1-20905] - (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: DAGLA Antibody [NBP1-20905] - (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - (5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DAGLA Antibody Summary

Immunogen
Peptide with sequence C-PAKQDELVISAR corresponding to C-Terminus according to NP_006124.1.
Epitope
C terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DAGLA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3-1 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 125 kDa band observed in human liver lysates (calculated MW of 115 kDa band according to NP_006124.1). Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than WB.
Publications
Read Publication using NBP1-20905.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.This batch is not expected to cross-react with Mouse and Rat in Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DAGLA Antibody

  • C11orf11
  • DAGL(ALPHA)
  • DAGLALPHA
  • DGL-alpha
  • diacylglycerol lipase, alpha
  • EC 3.1.1.-
  • KIAA0659
  • Neural stem cell-derived dendrite regulator
  • NSDDRchromosome 11 open reading frame 11
  • sn1-specific diacylglycerol lipase alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAGLA Antibody (NBP1-20905)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DAGLA
Entrez
Uniprot