DAGLA Antibody

Western Blot: DAGLA Antibody [NBP1-20905] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - Staining of paraffin embedded Human Breast. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - Staining of paraffin embedded Human Pancreas. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DAGLA Antibody Summary

• Description: Novus Biologicals Goat DAGLA Antibody (NBP1-20905) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DAGLA Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PAKQDELVISAR corresponding to C-Terminus according to NP_006124.1.
• Epitope: C terminus
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DAGLA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 125 kDa band observed in human liver lysates (calculated MW of 115 kDa band according to NP_006124.1). Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than WB.

Reactivity Notes

This batch is not expected to cross-react with Mouse and Rat in Western blot.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAGLA Antibody

• C11orf11
• DAGL(ALPHA)
• DAGLA
• DAGLALPHA
• DAGL-alpha
• DGL-alpha
• diacylglycerol lipase, alpha
• EC 3.1.1.-
• EC 3.1.1.116
• KIAA0659
• Neural stem cell-derived dendrite regulator
• NSDDR
• NSDDRchromosome 11 open reading frame 11
• sn1-specific diacylglycerol lipase alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAGLA Antibody (NBP1-20905)(1)

Publication using NBP1-20905

• Bisogno T, Howell F, Williams G et al. Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain. J Cell Biol 2003-11-10 [PMID: 14610053]

Bioinformatics

• Gene Symbol: DAGLA
• Entrez:
  • 747(Human)
  • 269060(Mouse)
  • 309207(Rat)
• Uniprot:
  • NP_006124.1(Human)