DACT3/Dapper 3 Antibody - BSA Free

Western Blot: DACT3/Dapper 3 Antibody [NBP1-76340] - Rat brain tissue lysate with Dact3 antibody at 1 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DACT3/Dapper 3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DACT3/Dapper 3 Antibody - BSA Free (NBP1-76340) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Dact3. The immunogen is located within amino acids 370 - 420 of Dact3. Amino Acid Squence: SVEQSPPRERPRAAGRR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DACT3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1 ug/ml
• Control Peptide:

  DACT3/Dapper 3 Peptide (NBP1-76340PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for DACT3/Dapper 3 Antibody - BSA Free

• Antagonist of beta-catenin Dapper homolog 3
• arginine rich region 1
• Arginine-rich region 1 protein
• DACT3
• Dapper 3
• dapper homolog 3
• dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis)
• MGC15476
• RRR1

Background

The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact3, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact3 is expressed in the ventral region of maturing somites, limb bud and branchial arch mesenchyme, embryonic CNS, and the adult brain. Recent evidence shows that Dact3 acts as a negative regulator Wnt/beta-catenin signaling that is repressed at the transcriptional level in colorectal cancer and this repression is associated with bivalent histone modifications. This repression can be reversed by pharmacological agents that targets both histone methylation and deacetylation, suggesting that Dact3 may be a potential target for therapeutic treatment of this cancer. At least three isoforms of Dact3 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DACT3
• Uniprot:
  • Q96B18(Human)