DAB2 Antibody (883216) (MAB8064)

Images

 
Western blot shows lysates of A172 human glioblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DAB2 Monoclonal Antibody (Catalog # ...read more
DAB2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DAB2 Monoclonal Antibody (Catalog # MAB8064) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
DAB2 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human DAB2 Monoclonal Antibody (Catalog # MAB8064) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC
Clone
883216
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

DAB2 Antibody (883216) Summary

Immunogen
E. coli-derived recombinant human DAB2
Lys630-Ala770
Accession # P98082
Specificity
Detects human DAB2 in ELISA and Western Blot.
Source
N/A
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
DAB2
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DAB2 Antibody (883216)

  • DAB2
  • Differentially-expressed protein 2
  • disabled (Drosophila) homolog 2 (mitogen-responsive phosphoprotein)
  • disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
  • Disabled-2
  • DOC2
  • DOC-2DOC2disabled homolog 2
  • FLJ26626
  • mitogen-responsive phosphoprotein

Background

DAB2 (Drosophila disabled homolog 2; also DOC-2/Differentially-expressed in Ovarian Carcinoma and p96) is a cytoplasmic phosphoprotein. Although the predicted MW is 82 kDa, it runs anomalously at 95-105 kDa in SDS-PAGE. It is widely expressed, being found in ovarian cuboidal epithelium, megakaryocytes, fibroblasts, macrophages, breast epithelium and renal proximal tubule cells. Reduced expression levels are associated with tumor development. DAB2 has multiple functions, but is typically described as an adaptor protein (i.e.-one that supports the apposition of two interacting molecules) involved in intracellular trafficking. Molecules reported to interact with DAB2 include megalin, SMAD2 plus TGF-beta RI/II, axin and Dvl3. With respect to trafficking, DAB2 reportedly binds to AP-2, clathrin, LRP6 and myosin VI, four molecules associated with the endocytic process. Human DAB2 is 770 amino acids (aa) in length. It contains one pleckstrin homology-like domain that binds phosphotyrosine (aa 42-170) and a C-terminal Pro-rich region that binds SH3 domains (aa 600-630). There are two isoform variants. One is 80-82 kDa in size and shows a deletion of aa 230-447. The second exhibits a short deletion of aa 209-229. Over aa 630-770, human DAB2 shares 76% aa sequence identity with mouse DAB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DAB2
Entrez
Uniprot

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