DAAM1 Antibody (5D3)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 5D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DAAM1 Antibody (5D3) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone 5D3 was generated from a recombinant protein that included amino acid residues from the N-terminal region of human DAAM1. This sequence has less than 50% homology to the N-terminal region of DAAM2. [UniProt# Q9Y4D1]
• Specificity: The antibody detects a 125 kDa protein corresponding to the apparent molecular mass of DAAM1 on SDS-PAGE immunoblots of human A431, K562, and mouse C2C12 cells.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAAM1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:500
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for DAAM1 Antibody (5D3)

• disheveled-associated activator of morphogenesis 1
• dishevelled associated activator of morphogenesis 1
• dishevelled-associated activator of morphogenesis 1
• KIAA0666FLJ41657

Background

Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAAM1