Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Cytosolic Sulfotransferase 2B1/SULT2B1 isoform 1
  Asp2-Ser365
  Accession # O00204
• Specificity: Detects human SULT2B1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SULT1B1, rhSULT1A1, rhSULT2A1, rhSULT4A1, and rhSULT1E1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody [Alexa Fluor® 647]

• Alcohol sulfotransferase
• Cytosolic Sulfotransferase 2B1
• EC 2.8.2
• EC 2.8.2.2
• HSST2
• HSST2sulfotransferase family cytosolic 2B member 1
• Hydroxysteroid sulfotransferase 2
• ST2B1
• Sulfotransferase 2B1
• sulfotransferase family, cytosolic, 2B, member 1
• SULT2B1

Background

SULT2B1 (Sulfotransferase 2B1; also ST2B1b and alcohol sulfotransferase) is a 45-48 kDa member of the sulfotransferase 1 family of enzymes. SULT2B1 is widely expressed, being found in platelets, stratum granulosum keratinocytes, breast and prostatic epithelium, and syncytiotrophoblast cells. SULT2B1 catalyzes the sulfonation of DHEA, a precursor for sex steroids, and cholesterol, which supports stratification of the epidermis. Human SULT2B1 is 365 amino acids (aa) in length. There are PAPS binding sites between aa 70-75 and 147-155, a myristoylation motif between aa 255-259, and a Pro-rich region between aa 305-364 that may extend enzyme half-life. There is one 43 kDa splice form (ST2B1a) that shows an eight aa substitution for aa 1-23. It is expressed in fetal brain and generates pregnenolone sulfate, a steroid that modulates neurotransmitters. Full-length human SULT2B1b shares only 71% aa identity with mouse SULT2B1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.