Cytokeratin, pan Antibody (SPM115 + SPM116)

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - Formalin-fixed, paraffin-embedded human Breast carcinoma stained with pan cytokeratin Monoclonal antibody cocktial (SPM115+SPM116)

Flow (Intracellular): Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - An intracellular stain was performed on HeLa cells with Cytokeratin, pan Antibody (SPM115 + SPM116) NBP2-34394APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin. Image using the Allophycocyanin format of this antibody.

Flow (Intracellular): Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - An intracellular stain was performed on HeLa cells with Cytokeratin, pan Antibody (SPM115 + SPM116) NBP2-34394APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin. Image using the Azide and BSA Free format of this antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: SPM115 + SPM116
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin, pan Antibody (SPM115 + SPM116) Summary

• Immunogen: Human epidermal keratin
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Flow (Intracellular)
• Application Notes: Human Chromosome Location: 17q21.2 (KRT77) & 12q13.13 (KRT76)
  Mol. Weight of Antigen: 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)
  Immunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT1