Cytokeratin, HMW Antibody - BSA Free

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody [NBP1-81648] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody [NBP1-81648] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody [NBP1-81648] - Staining of human endometrium shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody [NBP1-81648] - Staining of human cerebral cortex shows no positivity in neurons as expected.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin, HMW Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cytokeratin, HMW Antibody - BSA Free (NBP1-81648) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-Cytokeratin, HMW Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SGSGYGGVSSGSTGGRGSSGSYQSSSSGSRLGGAGSISVSHSGMGSSSGSIQTSGGSGYKSGGGGSTSIRFSQTTSSSQHSSTK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT76
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Cytokeratin, HMW Protein (NBP1-81648PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Cytokeratin, HMW Antibody - BSA Free

• CK-2P
• cytokeratin 2
• Cytokeratin-2P
• HUMCYT2A
• K2P
• K76
• keratin 76
• keratin, type II cytoskeletal 2 oral
• Keratin-76
• KRT2Bcytokeratin-2P
• KRT2Pkeratin 2p
• KRT76
• Type-II keratin Kb9

Background

KRT76, also known as Keratin, type II cytoskeletal 2 oral, is a 638 amino acid that is approx. 66 kDa, belongs to the intermediate filament family responsible for the structural integrity of epithelial cells, subdivided into epithelial keratins and hair keratins, and probably contributes to terminal cornification. Studies are being performed in relation to this protein and obstructive jaundice, pharyngitis, jaundice, cervical cancer, neuronitis, colorectal cancer, cervicitis, interferon and hepatitis. This protein has been shown to have interactions with ATG13, PDPK1, ULK2, GABARAP, and PRKAA1 in cytoskeleton organization pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin, HMW Antibody (NBP1-81648)(1)

Publication using NBP1-81648

• DiTommaso T, Cottle DL, Pearson HB et al. Keratin 76 Is Required for Tight Junction Function and Maintenance of the Skin Barrier. PLoS Genet 2014-10-01 [PMID: 25340345] (IF/IHC, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: KRT76