Cytokeratin 8 Antibody (TS1) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Ft(-), Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
TS1
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

Cytokeratin 8 Antibody (TS1) [Janelia Fluor® 549] Summary

Immunogen
Keratin preparation from a human carcinoma
Localization
Cytoplasmic
Specificity
Epitope of this MAb is located between aa343-357 (ELAIKDANAKLSELE). Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Cytokeratin 8 Antibody (TS1) [Janelia Fluor® 549]

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin 8
  • Cytokeratin-8
  • EndoA
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • KRT8
  • Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Cytokeratin 8 Antibody (NBP2-34501JF549) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34501AF350
Alexa Fluor 405 NBP2-34501AF405
Alexa Fluor 488 NBP2-34501AF488
Alexa Fluor 532 NBP2-34501AF532
Alexa Fluor 594 NBP2-34501AF594
Alexa Fluor 647 NBP2-34501AF647
Alexa Fluor 700 NBP2-34501AF700
Alexa Fluor 750 NBP2-34501AF750
Allophycocyanin NBP2-34501APC
Biotin NBP2-34501B
DyLight 350 NBP2-34501UV
DyLight 405 NBP2-34501V
DyLight 488 NBP2-34501G
DyLight 550 NBP2-34501R
DyLight 594 NBP2-34501DL594
DyLight 650 NBP2-34501C
DyLight 680 NBP2-34501FR
DyLight 755 NBP2-34501IR
FITC NBP2-34501F
HRP NBP2-34501H
Janelia Fluor 549 NBP2-34501JF549
Janelia Fluor 646 NBP2-34501JF646
PE NBP2-34501PE

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Blogs on Cytokeratin 8.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT8