Cytokeratin 8 Antibody (LP3K) [Alexa Fluor® 488]

Human peripheral blood mononuclear cells (PBMCs) (upper panel, negative control) and HT-29 human colon adenocarcinoma cell line (lower panel, positive control) were stained with Mouse Anti-Human Cytokeratin 8 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC3165G, filled histograms) or isotype control antibody (Catalog # IC002G, open histograms). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: LP3K
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Cytokeratin 8 Antibody (LP3K) [Alexa Fluor® 488] Summary

• Immunogen: SV-40 transformed human neonatal keratinocyte extract
• Specificity: Detects human Cytokeratin 8 in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytokeratin 8 Antibody (LP3K) [Alexa Fluor® 488]

• CARD2
• CK8
• CK-8
• CYK8
• Cytokeratin 8
• Cytokeratin-8
• EndoA
• K2C8
• K8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• KRT8
• Type-II keratin Kb8

Background

Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 8 Antibody (IC3165G)(2)

Publications using IC3165G

• Huang-Chiao Huang, Imran Rizvi, Joyce Liu, Sriram Anbil, Ashish Kalra, Helen Lee et al. Photodynamic Priming Mitigates Chemotherapeutic Selection Pressures and Improves Drug Delivery Cancer Research 2018-01-15 [PMID: 29187403]
• Huang-Chiao Huang, Imran Rizvi, Joyce Liu, Sriram Anbil, Ashish Kalra, Helen Lee et al. Photodynamic Priming Mitigates Chemotherapeutic Selection Pressures and Improves Drug Delivery Cancer Research 2018-01-15 [PMID: 29187403] (Immunohistochemistry, Xenograft)

Bioinformatics

• Gene Symbol: KRT8