Cytokeratin 8 Antibody (EP1628Y)

Product Discontinued

Cytokeratin 8 Antibody (EP1628Y) Summary

• Immunogen: A synthetic pathway corresponding to residues on the C-terminus of human CK8 was used as an immunogen.
• Marker: Epithelial Cell Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT8
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-500
  • Immunoprecipitation 1:70
  • Western Blot 1:25000-50000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cytokeratin 8 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Cytokeratin 8 Antibody (EP1628Y)

• CARD2
• CK8
• CK-8
• CYK8
• Cytokeratin 8
• Cytokeratin-8
• EndoA
• K2C8
• K8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• KRT8
• Type-II keratin Kb8

Background

Keratins, the intermediate filament proteins of epithelial cells, connect to desmosomes, the cell-cell adhesion structures at the surface membrane. Among the various intermediate filament (IF) proteins, cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells (1). Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine (2). While K8/K18 are essential for maintaining structural integrity, there is accumulating evidence indicating that they also exert non-mechanical functions. It has been reported that K8/K18-free hepatocytes from K8-null mice are more sensitive to Fas-mediated apoptosis, in line with an increased Fas density at the cell surface and an altered c-Flip regulation of the anti-apoptotic ERK1/2 signaling pathway (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 8 Antibody (NB110-56919)(2)

Publications using NB110-56919

• Luo Rongcan, Wang Yongqing, Xu Peng et al. Hypoxia-inducible miR-210 contributes to preeclampsia via targeting thrombospondin type I domain containing 7A. Scientific Reports 2016-01-01 [PMID: 26796133] (IHC-P, Human)
• Hobson Julia, Gummadidala Phani, Silverstrim Brian et al. Acute inflammation induced by the biopsy of mouse mammary tumors promotes the development of metastasis. Breast Cancer Research and Treatment 2013-01-01 [PMID: 23715631] (IF/IHC, IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: KRT8
• Entrez:
  • 3856(Human)
  • 16691(Mouse)
  • 25626(Rat)
• Uniprot:
  • P05787(Human)