Cytokeratin 8/18 Antibody (5D3)

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139] - Formalin fixed paraffin embedded human skin stained with Cytokeratin 8/18 antibody (NB120-17139).

• Reactivity: Hu, Mu
• Applications: IHC
• Clone: 5D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cytokeratin 8/18 Antibody (5D3) Summary

• Immunogen: Cytokeratins from the human breast carcinoma cell line MCF-7.
• Localization: Cytoplasmic
• Specificity: This antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa). It stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT8
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 11277418). Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Cytokeratin 8/18 Antibody (5D3)

• CARD2
• CK8
• CK-8
• CYK8cytokeratin 8
• Cytokeratin-8
• K2C8
• K8cytokeratin-8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• Type-II keratin Kb8

Background

Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with Cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 8/18 Antibody (NB120-17139)(5)

Publications using NB120-17139

• Busch A, Bauer L, Wardelmann E et al. Prognostic relevance of epithelial-mesenchymal transition and proliferation in surgically treated primary parotid gland cancer. J. Clin. Pathol. 2016-10-11 [PMID: 27729431] (IF/IHC, Human)
• Martin CA, Salomoni PD, Badran AF. Cytokeratin immunoreactivity in mouse tissues: study of different antibodies with a new detection system. Appl Immunohistochem Mol Morphol. 2001-03-01 [PMID: 11277418] (IHC-P, Mouse)
  
  Details:
  IHC (paraffin), mouse tissues including lung, kidney, liver, stomach, and small and large intestine: Representative data shown in Fig 3 (colon mucosa).
• Chang Kuang-Leei Chao Wan-Ru Han Chih-Ping. Anticytokeratin (CAM5.2) Reagent Identifies Cytokeratins 7 and 9, Not Cytokeratin 18. CHEST.
  
  Details:
  A shoRat commentary on cytokeratin antibodies.
• Chang Kuang-Leei Chao Wan-Ru Han Chih-Ping. Anticytokeratin (CAM5.2) Reagent Identifies Cytokeratins 7 and 9, Not Cytokeratin 18. Chest 2014-06-04 [PMID: 24889460]

Bioinformatics

• Gene Symbol: KRT8
• Entrez:
  • 3856(Human)