Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted [NBP2-48139] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/903)

Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Flow Cytometric Analysis of trypsinized methanol-fixed HeLa cells using Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

• Reactivity: Hu, Ma(-), Mu(-), Rb(-), Rt(-)
• Applications: IHC
• Clone: KRT7/903
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant full-length human Cytokeratin 7 protein (Uniprot: P08729)
• Localization: Cytoplasmic
• Marker: Glandular and Transitional Epithelial Marker
• Specificity: Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT7
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Hu-chromosome location: 12q13.13 Immunohistochemistry-Paraffin 1 - 2 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Mouse, Rat, Ferret or Rabbit.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted

• CK7
• CK-7
• Cytokeratin 7
• cytokeratin-7
• K2C7
• K7
• K7keratin, 55K type II cytoskeletal
• keratin 7
• keratin, type II cytoskeletal 7
• keratin-7
• MGC129731
• MGC3625
• sarcolectin
• SCLkeratin, simple epithelial type I, K7
• type II mesothelial keratin K7
• Type-II keratin Kb7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT7