Cytokeratin 7 Antibody (KRT7/760 + KRT7/903)

Images

 
Western Blot: Cytokeratin 7 Antibody (KRT7/760 + KRT7/903) [NBP2-44818] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (KRT7/760 + KRT7/903).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/760 + KRT7/903) [NBP2-44818] - Immunofluorescence Analysis of HeLa cells labeling Cytokeratin 7 with Cytokeratin 7 Antibody (KRT7/760 + KRT7/903) ...read more
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + KRT7/903) [NBP2-44818] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760 + KRT7/903)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + KRT7/903) [NBP2-44818] - Human Endometrial Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760 + KRT7/903)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + KRT7/903) [NBP2-44818] - Human Lung Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760 + KRT7/903)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
KRT7/760 + KRT7/903
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 7 Antibody (KRT7/760 + KRT7/903) Summary

Immunogen
Recombinant full-length human KRT7 protein
Localization
Cytoplasmic
Marker
Glandular and Transitional Epithelial Marker
Specificity
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Isotype
IgG1 Kappa/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 12q13.13
Immunohistochemistry-Paraffin 1 - 2 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 7 Antibody (KRT7/760 + KRT7/903)

  • CK7
  • CK-7
  • cytokeratin 7
  • cytokeratin-7
  • K2C7
  • K7keratin, 55K type II cytoskeletal
  • keratin 7
  • keratin, type II cytoskeletal 7
  • keratin-7
  • MGC129731
  • MGC3625
  • Sarcolectin
  • SCLkeratin, simple epithelial type I, K7
  • type II mesothelial keratin K7
  • Type-II keratin Kb7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 7 Antibody (NBP2-44818) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47942AF350
Alexa Fluor 405 NBP2-47942AF405
Alexa Fluor 488 NBP2-47942AF488
Alexa Fluor 532 NBP2-47942AF532
Alexa Fluor 594 NBP2-47942AF594
Alexa Fluor 647 NBP2-47942AF647
Alexa Fluor 700 NBP2-47942AF700
Alexa Fluor 750 NBP2-47942AF750
Allophycocyanin NBP2-47942APC
Biotin NBP2-47942B
DyLight 350 NBP2-47942UV
DyLight 405 NBP2-47942V
DyLight 488 NBP2-47942G
DyLight 550 NBP2-47942R
DyLight 594 NBP2-47942DL594
DyLight 650 NBP2-47942C
DyLight 680 NBP2-47942FR
DyLight 755 NBP2-47942IR
FITC NBP2-47942F
HRP NBP2-47942H
Janelia Fluor 549 NBP2-47942JF549
Janelia Fluor 646 NBP2-47942JF646
PE NBP2-47942PE
PerCP NBP2-47942PCP

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Bioinformatics

Gene Symbol KRT7
Uniprot
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