Cytokeratin 6a Antibody (rKRT6A/2100)

Images

 
Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (rKRT6A/2100) [NBP3-07715] - Formalin-fixed, paraffin-embedded human pancreas stained with Cytokeratin 6a Recombinant Mouse Monoclonal Antibody (rCytokeratin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P
Clone
rKRT6A/2100
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 6a Antibody (rKRT6A/2100) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant full-length human Cytokeratin 6a protein (Uniprot: P02538)
Localization
Cytoplasmic
Marker
Basal Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT6A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08749)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 6a Antibody (rKRT6A/2100)

  • Allergen Hom s 5
  • CK6A
  • CK-6A
  • CK6C
  • CK6D
  • CK-6D
  • cytokeratin 6A
  • cytokeratin 6C
  • cytokeratin 6D
  • Cytokeratin-6A
  • Cytokeratin-6D
  • K6A
  • K6C
  • K6D
  • keratin 6A
  • keratin 6C
  • keratin 6D
  • keratin, epidermal type II, K6A
  • keratin, type II cytoskeletal 6A
  • Keratin-6A
  • KRT6C
  • KRT6D
  • Type-II keratin Kb6

Background

The human type II Cytokeratin 6 (CK6; 56 kDa) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. It has been demonstrated that CK6 isoform, CK6a, is clearly the dominant CK6 isoform in skin tissue samples and cultured epithelial cell lines and that the various isoforms are differentially regulated within and between epithelial tissue types (1). The murine genome is known to have two keratin 6 (CK6) genes, mouse CK6, CK6a and MK6b. These genes display a complex expression pattern with constitutive expression in the epithelia of oral mucosa, hair follicles, and nail beds (2). Pachyonychia congenital (PC) is characterized by hypertrophic nail dystrophy and associated ectodermal features. PC-1 subtype is associated with mutations in keratins 6a or 16, whereas PC-2 subtype is linked to mutations in keratins 6b or 17 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KRT6A
Uniprot