Cytokeratin 20 Antibody

Product Discontinued

Cytokeratin 20 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids C-terminus of Human Cytokeratin 20.
• Specificity: This antibody detects endogenous levels of Keratin 20 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT20
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Cytokeratin 20 (D409) pAb in extracts from Hela cells.
  Immunohistochemistry (IHC) analyzes of Cytokeratin 20 (D409) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytokeratin 20 Antibody

• CD20
• CK20
• CK-20
• cytokeratin-20
• K20cytokeratin 20
• keratin 20
• keratin, type I cytoskeletal 20
• keratin-20
• KRT21
• MGC35423
• Protein IT

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins assemble into filaments, forming heterodimers of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles, which make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). Moll, R. et al. (1982) Cell 31, 11-24. Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613. Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354. Lane, E.B. and McLean, W.H. (2004) J. Pathol. 204, 355-366. Zatloukal, K. et al. (2004) J. Pathol. 204, 367-376. Owens, D.W. and Lane, E.B. (2004) J. Pathol. 204, 377-385.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT20
• Entrez:
  • 54474(Human)
  • 286912(Rat)