Cytokeratin 19 Antibody (SPM561)

Western Blot: Cytokeratin 19 Antibody (SPM561) [NBP2-34379] - Western Blot Analysis of HepG2 cell lysate using Cytokeratin 19 Antibody (SPM561).

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (SPM561) [NBP2-34379] - Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19 using Cytokeratin 19 Antibody (SPM561) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM561) [NBP2-34379] - Formalin-fixed, paraffin-embedded human Bladder carcinoma stained with cytokeratin 19 Monoclonal Antibody (SPM561).

Flow Cytometry: Cytokeratin 19 Antibody (SPM561) [NBP2-34379] - Flow Cytometric Analysis of MeOH-fixed MCF-7 cells using Cytokeratin 19 Antibody (SPM561) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometry: Cytokeratin 19 Antibody (SPM561) [NBP2-34379] - An intracellular stain was performed on HeLa cells with Cytokeratin 19 Antibody (SPM561) NBP2-34426APC and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: SPM561
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 19 Antibody (SPM561) Summary

• Immunogen: Human mammary epithelial organoids
• Localization: Cytoplasmic
• Marker: Pancreatic Stem Cell Marker
• Specificity: This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT19
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug
• Application Notes: ELISA (For coating, order NBP2-34426 (BSA free version of NBP2-34379)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 19 Antibody (SPM561)

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 19 Antibody (NBP2-34379)(1)

Publication using NBP2-34379

• Singh V, Yeoh BS, Chassaing B et al. Dysregulated Microbial Fermentation of Soluble Fiber Induces Cholestatic Liver Cancer. Cell. Oct 18 2018 [PMID: 30340040] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: KRT19
• Uniprot:
  • P08727()