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Cytokeratin 19 Antibody (SPM266)

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Western Blot: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (SPM266).
Western Blot: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (SPM266).
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with cytokeratin 19 Monoclonal Antibody (SPM266).
Flow Cytometry: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Flow Cytometric Analysis of Methanol-fixed MCF-7 cells. Cytokeratin 19 Antibody (SPM266) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control ...read more
Western Blot: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 MAb (SPM266).
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 MAb (SPM266).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
SPM266
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34405AF350
Alexa Fluor 405
NBP2-34405AF405
Alexa Fluor 488
NBP2-34405AF488
Alexa Fluor 532
NBP2-34405AF532
Alexa Fluor 594
NBP2-34405AF594
Alexa Fluor 647
NBP2-34405AF647
Alexa Fluor 700
NBP2-34405AF700
Alexa Fluor 750
NBP2-34405AF750
Allophycocyanin
NBP2-34405APC
Biotin
NBP2-34405B
DyLight 350
NBP2-34405UV
DyLight 405
NBP2-34405V
DyLight 488
NBP2-34405G
DyLight 550
NBP2-34405R
DyLight 594
NBP2-34405DL594
DyLight 650
NBP2-34405C
DyLight 680
NBP2-34405FR
DyLight 755
NBP2-34405IR
FITC
NBP2-34405F
HRP
NBP2-34405H
Janelia Fluor 549
NBP2-34405JF549
Janelia Fluor 646
NBP2-34405JF646
PE
NBP2-34405PE
PerCP
NBP2-34405PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-34405-0.1 mg
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Cytokeratin 19 Antibody (SPM266) Summary

Immunogen
Human breast cancer MCF-7 cells
Localization
Cytoplasmic
Marker
Pancreatic Stem Cell Marker
Specificity
This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT19
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug
Application Notes
ELISA (For coating, order NBP2-34405 (BSA free version of NBP2-34357))
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin 19 Antibody (SPM266)

  • 40-kDa keratin intermediate filament
  • CK19
  • CK-19
  • Cytokeratin 19
  • EndoC
  • K19
  • K1CS
  • keratin 19
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • keratin-19
  • Krt19
  • MGC15366

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
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Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).
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Western blot shows lysates of LNCaP human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CEACAM‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CEACAM‑7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
CEACAM‑7 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human CEACAM‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4478) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of Cytokeratin 20 on different cell lysates using anti-Cytokeratin 20 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo Lane 2: HCT116 Lane 3: CRC
Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of paraffin-embedded rat small intestine tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin.
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Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
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alpha-Fetoprotein/AFP ...
MAB1368
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[Unconjugated]
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Product General Protocols

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FAQs for Cytokeratin 19 Antibody (NBP2-34357) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34405AF350
Alexa Fluor 405 NBP2-34405AF405
Alexa Fluor 488 NBP2-34405AF488
Alexa Fluor 532 NBP2-34405AF532
Alexa Fluor 594 NBP2-34405AF594
Alexa Fluor 647 NBP2-34405AF647
Alexa Fluor 700 NBP2-34405AF700
Alexa Fluor 750 NBP2-34405AF750
Allophycocyanin NBP2-34405APC
Biotin NBP2-34405B
DyLight 350 NBP2-34405UV
DyLight 405 NBP2-34405V
DyLight 488 NBP2-34405G
DyLight 550 NBP2-34405R
DyLight 594 NBP2-34405DL594
DyLight 650 NBP2-34405C
DyLight 680 NBP2-34405FR
DyLight 755 NBP2-34405IR
FITC NBP2-34405F
HRP NBP2-34405H
Janelia Fluor 549 NBP2-34405JF549
Janelia Fluor 646 NBP2-34405JF646
PE NBP2-34405PE
PerCP NBP2-34405PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol KRT19
Uniprot
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