Cytokeratin 19 Antibody (SB39g)

Images

 
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SB39g) [NBP1-28570] - Cell cytospin of the HCC38 pancreatic carcinoma cell line was incubated with purified SB39g anti-keratin monoclonal antibody. After ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, ICC
Clone
SB39g
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Cytokeratin 19 Antibody (SB39g) Summary

Immunogen
Recombinant C-terminal keratin 19
Marker
Epithelial Cell Marker
Specificity
Keratin 19
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
KRT19
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:4000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunocytochemistry
Reviewed Applications
Read 1 Review rated 4
using
NBP1-28570 in the following applications:

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 19 Antibody (SB39g)

  • CK19
  • CK-19
  • Cytokeratin 19
  • Cytokeratin-19,40-kDa keratin intermediate filament
  • EndoC
  • K19
  • K19cytokeratin-19
  • K1CS
  • keratin 19
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • keratin-19
  • Krt19
  • MGC15366

Background

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. (2-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 19 is the smallest human keratin, is found in many simple and some non-keratinizing stratified squamous epithelia, and is often co-expressed with keratin 7. (7,8) Cytokeratin 19 has been implicated as a marker for stem cells in some tissues, such as hair follicles in the skin. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. The degree of cytokeratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Cytokeratin 19 is also associated with malignant transformation in esophageal and pancreatic cancers. (9,10)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 19 Antibody (NBP1-28570) (0)

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Review for Cytokeratin 19 Antibody (NBP1-28570) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-28570:
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/21/2010
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Summary

ApplicationWestern Blot
Sample Testedmouse embryonic fibroblast cell line, Sample Amount: 40 ug
SpeciesMouse
LotF1306-VB39

Blocking

Blocking DetailsBlocking Buffer: 5% milk/TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: 2hrs, Incubation Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse IgG from SCBT, Secondary Ab Dilution Ratio: 1/3000

Details

Detection NotesDetection Method: ECL Plus, Specific Bands: 44 kDa, Non-Specific Bands: above 55 kDa and below 34 kDa

Product General Protocols

View specific protocols for Cytokeratin 19 Antibody (NBP1-28570): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 19 Antibody (NBP1-28570) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
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0
2
0
1
0

 
Anonymous
01/21/2010
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol KRT19
Entrez