Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (SB39g) [NBP1-28570] - Cell cytospin of the HCC38 pancreatic carcinoma cell line was incubated with purified SB39g anti-keratin monoclonal antibody. After washing, a 10ug/mL dilution of goat anti-mouse Ig-FITC was used for immunofluorescence staining. Positive cells were viewed by fluorescence microscopy.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SB39g) [NBP1-28570] - Human gastric cancer tissue was stained with Mouse IgG2a-HRP isotype control ( left) and Mouse Anti-Cytokeratin 19-HRP (right) followed by DAB and hematoxylin.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: SB39g
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.1 mg/ml

Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free (NBP1-28570) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant C-terminal keratin 19
• Marker: Epithelial Cell Marker
• Specificity: Keratin 19
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT19
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000-1:4000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Background

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. (2-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 19 is the smallest human keratin, is found in many simple and some non-keratinizing stratified squamous epithelia, and is often co-expressed with keratin 7. (7,8) Cytokeratin 19 has been implicated as a marker for stem cells in some tissues, such as hair follicles in the skin. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. The degree of cytokeratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Cytokeratin 19 is also associated with malignant transformation in esophageal and pancreatic cancers. (9,10)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Cytokeratin 19 Antibody (NBP1-28570) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 01/21/2010

Summary

• Application: Western Blot
• Sample Tested: mouse embryonic fibroblast cell line, Sample Amount: 40 ug
• Species: Mouse
• Lot: F1306-VB39

Bioinformatics

• Gene Symbol: KRT19