Cytokeratin 19 Antibody (Ks19.1)

Western Blot: Cytokeratin 19 Antibody (Ks19.1) [NBP2-44828] - Western Blot Analysis of Human Prostrate cancer PC-3 cell lysate using Cytokeratin 19 Antibody (Ks19.1)

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (Ks19.1) [NBP2-44828] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (Ks19.1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (Ks19.1) [NBP2-44828] - Human Ovarian Carcinoma stained with Cytokeratin 19 Ab (Ks19.1).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: Ks19.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 19 Antibody (Ks19.1) Summary

• Immunogen: Human breast cancer MCF-7 cells
• Localization: Cytoplasmic
• Marker: Pancreatic Stem Cell Marker
• Specificity: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT19
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 17q21.2
  Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 19 Antibody (Ks19.1)

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cytokeratin 19 Antibody (NBP2-44828). (Showing 1 - 1 of 1 FAQ).

1. Do you have the clone identity for this antibody, NBP2-44828-0.1mg? The clone that we have been using and publishing is Clone A53-B/A2.26. It says that it is also known as Ks19.1 but I would like to make sure that this is the same.
   • A53-B/A2.26 and Ks19.1 are indeed the same clone, so NBP2-44828-0.1mg should work well for you.

Bioinformatics

• Gene Symbol: KRT19
• Uniprot:
  • P08727()