Cytokeratin 19 Antibody (KRT19/800)

Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19 using Cytokeratin 19 Antibody (KRT19/800) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Formalin-fixed, paraffin-embedded Rat Colon stained with Cytokeratin 19 Antibody (KRT19/800).

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Colon stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Lung stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Stomach stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clone: KRT19/800
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 19 Antibody (KRT19/800) Summary

• Immunogen: Recombinant full-length human Cytokeratin 19 protein. (Uniprot: P08727 )
• Localization: Cytoplasmic
• Marker: Pancreatic Stem Cell Marker
• Specificity: Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT19
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47946)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 19 Antibody (KRT19/800)

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Background

Cytokeratin 19 (also known as Keratin, type I cytoskeletal 19) belongs to the keratin family which consists of fibrous proteins that make up the framework of epithelial cells. Cytokeratin 19 plays a role in the organization of myofibers. This protein is known to have interactions with ZNF638, FANCG, FAM107A, KRT15 and EXOC8. Cytokeratin 19 has been studied in relation to several disorders and diseases including chordoma, adenoma, hamartoma, adenoiditis, carcinoma, and liver disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT19
• Uniprot:
  • P08727()
  • P08727()