Cytokeratin 19 Antibody (BA17)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (BA17) [NBP2-34271] - Formalin-fixed, paraffin-embedded human colon stained with Cytokeratin 19 MAb (BA17)

Flow Cytometry: Cytokeratin 19 Antibody (BA17) [NBP2-34271] - An intracellular stain was performed on HepG2 cells with Cytokeratin 19 antibody (BA17) NBP2-34560APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Simple Western: Cytokeratin 19 Antibody (BA17) [NBP2-34271] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Cytokeratin 19 Antibody (BA17) [NBP2-34271] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

SDS-Page: Cytokeratin 19 Antibody (BA17) [NBP2-34271]

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PAGE
• Clone: BA17
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Cytokeratin 19 Antibody (BA17) Summary

• Immunogen: Human mammary epithelial organoids
• Localization: Cytoplasmic
• Marker: Pancreatic Stem Cell Marker
• Specificity: This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT19
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Simple Western 10 ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein
  • SDS-Page
• Application Notes: ELISA (For coating, order NBP2-34560 (BSA free version of NBP2-34271)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 19 Antibody (BA17)

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cytokeratin 19 Antibody (NBP2-34271). (Showing 1 - 1 of 1 FAQ).

1. We regularly use Cytokeratin 19 Antibody (BA17) [NBP2-34271][Allophycocyanin], however, the volumen on packaging of this antibody often changes (0.1 ml or 0.15 ml or 0.210 ml). So we want to know the exact concentration to ensure the consistency of the experiment.
   • All of our directly conjugated antibodies are made to order and we do not keep any in stock and the concentrations will always vary batch to batch. The final concentration will be determined upon completion and entered on the product label. Most concentrations will fall between 0.6 - 1.0 mg/ml; with the exception of APC which typically ranges between 0.3 - 0.5 mg/ml. Especially for APC conjugates, the concentrations can vary from lot to lot and thus there will be a different volume supplied. The vial labels will have the recommended dilutions to use based off the labs expertise (ie 1-2 ul per test, or 2-4 ul per test). Each vial will contact enough for roughly the same amount of tests.

Bioinformatics

• Gene Symbol: KRT19
• Uniprot:
  • P08727()