Cytokeratin 16 Antibody (SPM264)

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Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SPM264) [NBP3-07649] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 16 Mouse Monoclonal Antibody (SPM264).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
SPM264
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 16 Antibody (SPM264) Summary

Immunogen
Recombinant fragment from the C-terminal of human Cytokeratin 16 (Uniprot: P08779)
Localization
Cytoplasmic
Marker
Suprabasal Keratinocyte Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT16
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Frozen & formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08692)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 16 Antibody (SPM264)

  • CK16
  • CK-16
  • cytokeratin 16
  • cytokeratin-16
  • FNEPPK
  • focal non-epidermolytic palmoplantar keratoderma
  • K16
  • K1CP
  • keratin 16
  • keratin, type I cytoskeletal 16
  • keratin-16
  • KRT16A
  • NEPPK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT16
Uniprot