Cytokeratin 15 Antibody

Images

 
Western Blot: Cytokeratin 15 Antibody [H00003866-D01P] - Analysis of KRT15 expression in A-431.
Western Blot: Cytokeratin 15 Antibody [H00003866-D01P] - Analysis of KRT15 expression in transfected 293T cell line by KRT15 polyclonal antibody.Lane 1: KRT15 transfected lysate(49.20 KDa).Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cytokeratin 15 Antibody Summary

Immunogen
KRT15 (NP_002266.2, 1 a.a. - 456 a.a.) full-length human protein. MTTTFLQTSSSTFGGGSTRGGSLLAGGGGFGGGSLSGGGGSRSISASSARFVSSGSGGGYGGGMRVCGFGGGAGSVFGGGFGGGVGGGFGGGFGGGDGGLLSGNEKITMQNLNDRLASYLDKVRALEEANADLEVKIHDWYQKQTPTSPECDYSQYFKTIEELRDKIMATTIDNSRVILEIDNARLAADDFRLKYENELALRQGVEADINGLRRVLDELTLARTDLEMQIEGLNEELAYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMREQYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSLAETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIGIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT15
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
It has been used for WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cytokeratin 15 Antibody

  • CK15
  • CK-15
  • cytokeratin 15
  • Cytokeratin-15
  • K15keratin-15, basic
  • K1CO
  • keratin 15
  • keratin, type I cytoskeletal 15
  • Keratin-15
  • keratin-15, beta
  • KRTB
  • type I cytoskeletal 15

Background

A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues (1). Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath (2). In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT15
Uniprot