Cytokeratin 10 Antibody (SPM261) [PE]

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: SPM261
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

Cytokeratin 10 Antibody (SPM261) [PE] Summary

• Immunogen: Skin extract of a human Psoriasis patient
• Localization: Cytoplasmic
• Marker: Suprabasal Epithelial Marker
• Specificity: This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8-9, for 10-20 min followed by cooling at RT for 20 minutes)
• Theoretical MW: 56.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Cytokeratin 10 Antibody (SPM261) [PE]

• BCIE
• BIE
• CK10
• CK-10
• cytokeratin 10
• Cytokeratin-10
• EHK
• K10keratosis palmaris et plantaris
• Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris)
• keratin 10
• keratin, type I cytoskeletal 10
• keratin-10
• KPP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT10