Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free [NBP3-08475] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 1 Mouse Monoclonal Antibody (KRT1/1840).

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free [NBP3-08475] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 1 Mouse Monoclonal Antibody (KRT1/1840).

• Reactivity: Hu, Rt
• Applications: IHC
• Clone: KRT1/1840
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07776).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human Cytokeratin 1 protein (Uniprot: P04264)
• Localization: Cytoplasmic
• Marker: Suprabasal Keratinocyte Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 2-4 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free

• CK1
• CK-1
• cytokeratin 1
• Cytokeratin-1
• cytokeratin-1,67 kDa cytokeratin
• EHK
• EHK1
• epidermolytic hyperkeratosis 1
• EPPK
• Hair alpha protein
• K1
• keratin 1
• keratin, type II cytoskeletal 1
• Keratin-1
• KRT1A
• KRTA
• NEPPK
• Type-II keratin Kb1

Background

Belonging to the keratin family, Cytokeratin 1 plays an important role in cell binding. More specifically, this gene is expressed in the spinous and granular layers of the epidermis with keratin 10. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. This gene also regulates the activity of kinases such as PKC and SRC by binding to ITGB1 and the receptor of GNB2L1. Other biological processes include complement activation, lectin pathway, epidermis development, fibrinolysis, regulation of angiogenesis and response to oxidative stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT1