Cytohesin-1 Antibody (1D6) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Cytohesin-1 Antibody (1D6) [H00009267-M01] - Analysis of monoclonal antibody to PSCD1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (1D6) [H00009267-M01] - Analysis of monoclonal antibody to PSCD1 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.

Sandwich ELISA: Cytohesin-1 Antibody (1D6) [H00009267-M01] - Detection limit for recombinant GST tagged PSCD1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cytohesin-1 Antibody (1D6) - Azide and BSA Free Summary

• Immunogen: PSCD1 (AAH50452, 1 a.a. ~ 398 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEDDSYVPSDLTAEERQELENIRRRKQELLADIQRLKDEIAEVANEIENLGSTEERKNMQRNKQVAMGRKKFNMDPKKGIQFLIENDLLKNTCEDIAQFLYKGEGLNKTAIGDYLGERDEFNIQVLHAFVELHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCQCNNGVFQSTDTCYVLSFAIIMLNTSLHNPNVKDKPTVERFIAMNRGINDGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDSKKPNCFELYIPDNKDQVIKACKTEADGRVVEGNHTVYRISAPTPEEKEEWIKCIKAAISRDPFYEMLAARKKKVSSTKRH
• Specificity: PSCD1 - pleckstrin homology, Sec7 and coiled-coil domains 1(cytohesin 1)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYTH1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), immunofluorescence, and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cytohesin-1 Antibody (1D6) - Azide and BSA Free

• B2-1
• CYTH1
• cytoadhesin 1
• cytohesin 1
• Cytohesin1
• Cytohesin-1
• D17S811ECYTOHESIN-1
• FLJ34050
• FLJ41900
• homolog of secretory protein SEC7
• PH, SEC7 and coiled-coil domain-containing protein 1
• pleckstrin homology, Sec7 and coiled-coil domains 1
• PSCD1
• SEC7 homolog B2-1
• SEC7

Background

Pleckstrin homology, Sec7 and coiled/coil domains 1 (PSCD1) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The PSCD1 is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. PSCD1 protein is 83% homologous to PSCD2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYTH1
• Entrez:
  • 9267(Human)
• OMIM:
  • 182115(Human)
• Uniprot:
  • AAH50452(Human)