Cytochrome P450 4A Antibody

Western Blot. Cytochrome P450 4A Antibody [NBP2-16081] - Effect of fisetin on lipid formation and the expression of lipid metabolism-related protein in the liver of mice chronically fed ethanol for 4 weeks. Image submitted by a verified customer review .

Immunocytochemistry/Immunofluorescence: Cytochrome P450 4A Antibody [NBP2-16081] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Cytochrome P450 4A Antibody [NBP2-16081] - Human hepatoma, using CYP4A11 antibody(GTX114353) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: Cytochrome P450 4A Antibody [NBP2-16081] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cytochrome P450 4A Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CYP4A11. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane; Microsome membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP4A11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (88%), Bovine (83%). Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 4A Antibody

• 20-HETE synthase
• CP4Y
• CYP4A2
• cytochrome P450 4A11
• cytochrome P450, family 4, subfamily A, polypeptide 11
• cytochrome P450, subfamily IVA, polypeptide 11
• cytochrome P-450HK-omega
• cytochrome P450HL-omega
• fatty acid omega-hydroxylase
• lauric acid omega-hydroxylase
• P450HL-omega

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates medium-chain fatty acids such as laurate and myristate. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cytochrome P450 4A Antibody (NBP2-16081)(1)

Publication using NBP2-16081

• Sun Q, Zhang W, Zhong W et al. Dietary Fisetin Supplementation Protects Against Alcohol-Induced Liver Injury in Mice. Alcohol Clin Exp Res 2016-08-30 [PMID: 27575873] (WB, Mouse)

Review for Cytochrome P450 4A Antibody (NBP2-16081) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 09/20/2017

Summary

• Application: Western Blot
• Sample Tested: Liver
• Species: Mouse
• Lot: 40184

Bioinformatics

• Gene Symbol: CYP4A11
• Uniprot:
  • Q02928(Human)