![Western Blot: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 4A (CYP4A11) Rabbit mAb (NBP3-15847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cytochrome-P450-4A-Antibody-10D10W7-Western-Blot-NBP3-15847-img0004.jpg "Western Blot: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 4A (CYP4A11) Rabbit mAb (NBP3-15847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | 10D10W7 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit Cytochrome P450 4A Antibody (10D10W7) (NBP3-15847) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Cytochrome P450 4A (CYP4A11) (Q02928). QFPCPPSHWLFGHIQELQQDQELQRIQKWVETFPSACPHWLWGGKVRVQLYDPDYMKVILGRSDPKSHGSYRFLAPWIGYGLLLLNGQTWFQHRRMLTPAF |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CYP4A11 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol, 0.05% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CYP4A11 |
![Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded mouse kidney tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15847_rabbit-cytochrome-p450-4a-mab-10d10w7-72202512435322.jpg "Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded mouse kidney tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded rat kidney tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15847_rabbit-cytochrome-p450-4a-mab-10d10w7-7220251245339.jpg "Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded rat kidney tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded human liver tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15847_rabbit-cytochrome-p450-4a-mab-10d10w7-72202512435385.jpg "Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded human liver tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")