Cytochrome P450 2E1 Antibody

Western Blot: Cytochrome P450 2E1 Antibody [NBP1-52087] - Analysis of Cytochrome P450 2E1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-52087 at 0.05 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-52087] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cytochrome P450 2E1 Antibody Summary

• Description: Novus Biologicals Goat Cytochrome P450 2E1 Antibody (NBP1-52087) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Cytochrome P450 2E1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RKVIKNVAEVK corresponding to internal region according to NP_000764.1.
• Epitope: C-RKVIKNVAEVK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CYP2E1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.05 - 0.2 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human liver lysates (calculated MW of 56.8 kDa band according to NP_000764.1). IHC-P: Human liver shows ER-like staining in hepatocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 2E1 Antibody

• 4-nitrophenol 2-hydroxylase
• CPE1
• CYP2Ecytochrome P450 2E1
• CYPIIE1
• cytochrome P450, family 2, subfamily E, polypeptide 1
• cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
• Cytochrome P450-J
• EC 1.14.13.-
• EC 1.14.13.n7
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• microsomal monooxygenase
• P450C2E
• P450-J
• xenobiotic monooxygenase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 2E1 Antibody (NBP1-52087)(1)

Publication using NBP1-52087

• Niu Y, Yuan H, Leng W et al. CYP2E1 Rsa I/Pst I polymorphism and esophageal cancer risk: a meta-analysis based on 1,088 cases and 2,238 controls. Med Oncol 2011-03-01 [PMID: 20195803]

Bioinformatics

• Gene Symbol: CYP2E1
• Entrez:
  • 1571(Human)
• Uniprot:
  • NP_000764.1(Human)