Cytochrome P450 2E1 Antibody

Images

 
Western Blot: Cytochrome P450 2E1 Antibody [NBP1-52087] - Analysis of Cytochrome P450 2E1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-52087 at 0.05 ug/ml. Primary incubation was 1 hour. Detected by ...read more
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-52087] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Cytochrome P450 2E1 Antibody Summary

Immunogen
Peptide with sequence C-RKVIKNVAEVK corresponding to internal region according to NP_000764.1.
Epitope
C-RKVIKNVAEVK
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CYP2E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 - 0.2 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 50 kDa band observed in human liver lysates (calculated MW of 56.8 kDa band according to NP_000764.1). IHC-P: Human liver shows ER-like staining in hepatocytes.
Publications
Read Publication using NBP1-52087.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytochrome P450 2E1 Antibody

  • 4-nitrophenol 2-hydroxylase
  • CPE1
  • CYP2Ecytochrome P450 2E1
  • CYPIIE1
  • cytochrome P450, family 2, subfamily E, polypeptide 1
  • cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
  • Cytochrome P450-J
  • EC 1.14.13.-
  • EC 1.14.13.n7
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • microsomal monooxygenase
  • P450C2E
  • P450-J
  • xenobiotic monooxygenase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytochrome P450 2E1 Antibody (NBP1-52087)(1)

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Bioinformatics

Gene Symbol CYP2E1
Entrez
Uniprot