Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

ELISA: Human Cytochrome P450 1A1 ELISA Kit (Colorimetric) [NBP3-18022] - Samples were spiked with high concentrations of Human Cytochrome P450 1A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Cytochrome P450 1A1 ELISA Kit (Colorimetric) [NBP3-18022] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Cytochrome P450 1A1 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 6.10
• Intra-Assay: %CV < 5.95
• Spike Recovery: 83-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CYP1A1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

• AHH
• AHRR
• aryl hydrocarbon hydroxylase
• CP11
• CYP1
• CYP1A1
• CYPIA1
• cytochrome P1-450, dioxin-inducible
• Cytochrome P450 1A1
• Cytochrome P450 form 6
• cytochrome P450, family 1, subfamily A, polypeptide 1
• Cytochrome P450-C
• Cytochrome P450-P1
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• P1-450
• P450-C
• P450DX
• subfamily I (aromatic compound-inducible), polypeptide 1
• xenobiotic monooxygenase

Background

The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CYP1A1