![Western Blot: Cytochrome b5 type A Antibody (1A8) [H00001528-M06] - Analysis of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody (M06), clone 1A8. Lane 1: CYB5A transfected lysatE (15.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cytochrome-b5-type-A-Antibody-1A8-Western-Blot-H00001528-M06-img0003.jpg "Western Blot: Cytochrome b5 type A Antibody (1A8) [H00001528-M06] - Analysis of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody (M06), clone 1A8. Lane 1: CYB5A transfected lysatE (15.3 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 1A8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | CYB5A (AAH15182, 1 a.a. ~ 134 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED |
| Specificity | CYB5A - cytochrome b5 type A (microsomal) (1A8) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYB5A |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
| Publication using H00001528-M06 | Applications | Species |
|---|---|---|
| Ming DS, Pham S, Deb S et al. Pomegranate extracts impact the androgen biosynthesis pathways in prostate cancer models in vitro and in vivo. J Steroid Biochem Mol Biol 2014-02-22 [PMID: 24565566] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome b5 type A Antibody (H00001528-M06)Find related products by research area.
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![Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody (1A8) [H00001528-M06] - Analysis of monoclonal antibody to CYB5A on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Cytochrome-b5-type-A-Antibody-1A8-Immunohistochemistry-Paraffin-H00001528-M06-img0001.jpg "Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody (1A8) [H00001528-M06] - Analysis of monoclonal antibody to CYB5A on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml")
![ELISA: Cytochrome b5 type A Antibody (1A8) [H00001528-M06] - Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cytochrome-b5-type-A-Antibody-1A8-ELISA-H00001528-M06-img0004.jpg "ELISA: Cytochrome b5 type A Antibody (1A8) [H00001528-M06] - Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.")
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")