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Cystatin SN Antibody (111)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ELISA(Det)
Clone
111
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-89919AF350
Alexa Fluor 405
NBP2-89919AF405
Alexa Fluor 488
NBP2-89919AF488
Alexa Fluor 532
NBP2-89919AF532
Alexa Fluor 594
NBP2-89919AF594
Alexa Fluor 647
NBP2-89919AF647
Alexa Fluor 700
NBP2-89919AF700
Alexa Fluor 750
NBP2-89919AF750
Biotin
NBP2-89919B
DyLight 350
NBP2-89919UV
DyLight 405
NBP2-89919V
DyLight 488
NBP2-89919G
DyLight 550
NBP2-89919R
DyLight 594
NBP2-89919DL594
DyLight 650
NBP2-89919C
DyLight 680
NBP2-89919FR
DyLight 755
NBP2-89919IR
FITC
NBP2-89919F
HRP
NBP2-89919H
Janelia Fluor 549
NBP2-89919JF549
Janelia Fluor 646
NBP2-89919JF646
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Cystatin SN Antibody (111) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Cystatin SN (P01037-1; Met 1-Ser 141).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CST1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:10000
  • Sandwich ELISA Detection 1:1000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for Cystatin SN Antibody (111)

  • CST1
  • cystain-SA-I
  • cystatin 1
  • cystatin SA-I
  • Cystatin SN
  • cystatin-1
  • cystatin-SN
  • Salivary cystatin-SA-1
  • type 2 family

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Cystatin S
Antibody (OTI2H10) - ...
NBP2-70481
Western Blot: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - Analysis of extracts (10ug) from HeLa cell line by using anti-CST4 monoclonal antibody at 1:200 dilution.
Western Blot: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cystatin S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cystatin S.
Flow Cytometry: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Cystatin S antibody, and then analyzed by flow cytometry.
Species: Hu, Pm
Applications: CyTOF-ready, Flow, ICC/IF, WB
Cystatin SA
Antibody (OTI1D6)
NBP2-01747
Immunohistochemistry-Paraffin: Cystatin SA Antibody (1D6) [NBP2-01747] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CST2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cystatin SA Antibody (1D6) [NBP2-01747] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CST2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cystatin SA Antibody (1D6) [NBP2-01747] - CST2 Antibody (1D6) [NBP2-01747] - Staining of paraffin-embedded Human endometrium tissue using anti-CST2 mouse monoclonal antibody.
Species: Hu
Applications: IHC, IHC-P, WB
Cystatin C [HRP]
MSCTC0
(1 Review)
(28 Publications)
Species: Mu, Rt
Applications: ELISA
Cathepsin C/DPPI
Antibody [Unconjugate ...
AF1034
Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cathepsin C/DPPI at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cathepsin C/DPPI was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Western blot shows lysates of mouse liver tissue and mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin C/DPPI at approximately 20-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(10 Publications)
Species: Mu, Rt
Applications: IHC, Simple Western, WB
Cystatin D/Cst5
1202-PI
Cystatin D/Cst5
(3 Publications)
Species: Hu
Applications: InhibAct
Kininogen Antibody
NBP1-89993
Immunohistochemistry-Paraffin: Kininogen Antibody [NBP1-89993] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Kininogen Antibody [NBP1-89993] - Staining in human kidney and skeletal muscle tissues.. Corresponding Kininogen RNA-seq data are presented for the same tissues.
Western Blot: Kininogen Antibody [NBP1-89993] - Analysis in control (vector only transfected HEK293T lysate) and Kininogen over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Cystatin E/M/CST6
Antibody [Unconjugate ...
AF1286
Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cystatin E/M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1286) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of human skin tissue, loaded at 0.2 mg/mL. A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Cystatin E/M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1286) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
(1 Review)
(4 Publications)
Species: Hu
Applications: IHC, IP, Simple Western, WB
Cathepsin B
Antibody [Unconjugate
AF953
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin B at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cathepsin B knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Cathepsin B at approximately 25-45 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.New adjunct appears with knockout cell line.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin B at approximately 34 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(3 Reviews)
(18 Publications)
Species: Hu
Applications: IHC, IP, KO, Simple Western, WB
Lipocalin-1
Antibody (10B10)
NBP1-28872
Western Blot: Lipocalin-1 Antibody (10B10) [NBP1-28872] - analysis using anti-LCN1 monoclonal antibody against HEK293 (1) and LCN1-hIgGFc transfected HEK293 cell lysate(2).
Immunocytochemistry/Immunofluorescence: Lipocalin-1 Antibody (10B10) [NBP1-28872] - analysis of methanol-fixed HEK293 cells trasfected with LCN1-hIgGFc using anti-LCN1 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye
Immunohistochemistry-Paraffin: Lipocalin-1 Antibody (10B10) [NBP1-28872] - Immunohistochemical analysis of paraffin-embedded human Prostate tissues using LCN1 mouse mAb
(1 Review)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
CSTP1 Antibody
NBP1-88048
Western Blot: CSTP1 Antibody [NBP1-88048] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: CSTP1 Antibody [NBP1-88048] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Western Blot: CSTP1 Antibody [NBP1-88048] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Species: Hu
Applications: IHC, IHC-P, WB
STATH Antibody
NBP1-92450
Immunohistochemistry-Paraffin: STATH Antibody [NBP1-92450] - Staining of human salivary gland shows high expression.
Immunohistochemistry-Paraffin: STATH Antibody [NBP1-92450] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: STATH Antibody [NBP1-92450] - Staining in human salivary gland and skeletal muscle tissues using anti-STATH antibody. Corresponding STATH RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
GAL3ST1 Antibody
H00009514-B01P
Western Blot: GAL3ST1 Antibody [H00009514-B01P] - Analysis of GAL3ST1 expression in transfected 293T cell line by GAL3ST1 polyclonal antibody. Lane1:GAL3ST1 transfected lysate(46.53 KDa). Lane2:Non-transfected lysate.
(3 Publications)
Species: Hu, Mu
Applications: WB
Trypsin 3/PRSS3
Antibody [Unconjugate
AF3565
Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using 5 µg/mL Goat Anti-Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(1 Review)
(1 Publication)
Species: Mu
Applications: IHC, IP, WB
CASZ1 Antibody
NBP1-86617
Immunohistochemistry-Paraffin: CASZ1 Antibody [NBP1-86617] - Staining of human skin shows strong nuclear positivity in stratum granulosum.
Immunocytochemistry/Immunofluorescence: CASZ1 Antibody [NBP1-86617] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(5 Reviews)
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Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Recombinant Human
SLC35A1 GST (N-Term) ...
H00010559-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Applications: ELISA, AP, PA, WB
Rpn2 Antibody
NBP2-38323
Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.
Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex, kidney, liver and testis using Anti-PSMD1 antibody NBP2-38323 (A) shows similar protein distribution across tissues to independent antibody NBP2-38324 (B).
Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human testis.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Calpastatin
Antibody (OTI1E7)
NBP2-01819
Flow Cytometry: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpastatin antibody, and then analyzed by flow cytometry.
Western Blot: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpastatin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpastatin.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Calpastatin mouse monoclonal antibody.
Species: Hu
Applications: Flow, IHC, IHC-P, WB
HPRG Antibody
[Unconjugated]
AF1905
HPRG Antibody [Unconjugated]
(1 Publication)
Species: Mu
Applications: WB
Cystatin A Antibody
NBP1-86989
Immunocytochemistry/Immunofluorescence: Cystatin A Antibody [NBP1-86989] - Staining of human cell line hTCEpi shows localization to nucleus & cytosol.
Western Blot: Cystatin A Antibody [NBP1-86989] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: Cystatin A Antibody [NBP1-86989] - Staining of human skin shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB

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FAQs for Cystatin SN Antibody (NBP2-89919). (Showing 1 - 1 of 1 FAQ).

  1. I notice you sell both antibodies and recombinant protein for human cystatin SN. Could you perhaps develop an ELISA for this protein?
    • NBP1-55995 and H00001469-B01P are both suitable for use as antibodies in ELISA. While a kit is not in development, you could use these to make your own ELISA kit, as many of our users do.

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Bioinformatics

Gene Symbol CST1